Age-Dependent Honey Bee Appetite Regulation Is Mediated by Trehalose and Octopamine Baseline Levels

Simple Summary:& nbsp;Appetite regulation is an important function necessary to maintain energetic balance, but how honey bees accomplish this could vary as they age because they go through a number of behavioral and physiological changes during development. Here, we determine if the amount of trehalose, which is a sugar found in the hemolymph of honey bees, influences appetite levels and if this interacts with the octopamine neurotransmitter in the bee brain. To accomplish this, we decreased trehalose levels in the hemolymph by injecting an inhibitor of trehalose synthesis. In addition, we increased octopamine levels in the brain by injecting it with a syringe. We found that octopamine and trehalose interact to increase appetite in the two older age classes of bees, beyond just treating the bees with octopamine. The youngest age class did not respond to either treatment. Our results suggest that older honey bees may have an alternative pathway for regulating appetite that uses sugar levels in their hemolymph to communicate to the brain how hungry they are and that octopamine is responsible for elevating appetite levels when the bee is hungry. This pathway is different from how vertebrates regulate their appetite levels based on glucose levels in the blood.