Published January 1, 2011 | Version v1
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Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides

  • 1. Hacettepe Univ, Dept Food Engn, TR-06800 Ankara, Turkey

Description

A competitive DNA hybridization assay based on the photoelectrochemistry of the semiconductor quantum dot-single stranded DNA conjugates (QD-ssDNA) was developed. Hybridization of QD-ssDNA with the capture probe DNA immobilized on the indium-tin oxide electrodes enables photocurrent generation when the electrochemical cell was illuminated with a light source. Upon the competition between QD-ssDNA and single-stranded target DNA, the photocurrent response decreased with the increase in the target DNA concentration. A linear relationship between the photocurrent and the target DNA concentration was obtained (R (2) = 0.991). The selectivity of system towards the target DNA was also demonstrated using non-complementary sample.

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