Published January 1, 2011
| Version v1
Journal article
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Toxoplasma gondii RH Ankara: Production of evolving tachyzoites using a novel cell culture method
Creators
- 1. Ege Univ, Sch Med, Dept Parasitol, TR-35100 Bornova, Turkey
- 2. Ege Univ, Sch Med, Dept Med Microbiol, TR-35100 Bornova, Turkey
Description
Toxoplasma gondii is one of the most researched parasite due to its easy growth both in vitro and in vivo. Tachyzoites, derived from mouse or rat peritoneum encounters ethical and economical problems when used for research or diagnostic purposes. Currently, research has focused on determining the most suitable cell culture environment to reach highest amount of viable tachyzoites with least host cell contamination. However, gene expression changes that take place throughout the adaptation of evolving T. gondii strains to continuous cell cultures appear as a problem.
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