TUBITAK Destekli Projelerden Çıkan Yayınlar Topluluğu
Published January 1, 2011 | Version v1
Journal article Open

First report on the phylogeny of bovine norovirus in Turkey

Creators

  • 1. Istanbul Univ, Dept Virol, Fac Vet, Istanbul, Turkey
  • 2. Istanbul Univ, Dept Food Hyg & Technol, Fac Vet, Istanbul, Turkey
  • 3. Cevre Ind Anal Lab, Istanbul, Turkey
  • 4. Univ Bristol, Langford Vet Serv, Bristol BS40 5DU, Avon, England
  • 5. Chonnam Natl Univ, Biotherapy Human Resources Ctr, Coll Vet Med, Kwangju 500757, South Korea

Description

Bovine norovirus (BoNoV) is an important cause of diarrhea in calves and has been reported in several countries. The aims of this study were to investigate for the first time the presence of norovirus in Turkish calves by real-time reverse transcription-polymerase chain reaction (qRT-PCR) and to determine the phylogeny of any circulating strains. Fecal samples from 70 diarrheic calves were collected and analysed by SYBR Green qRT-PCR. BoNoV was detected in fecal samples from six calves. The capsid gene was partially sequenced, and phylogenetic analysis was performed. This showed that the six Turkish BoNoVs clustered with the GIII-2 prototype.

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