Published January 1, 2011
| Version v1
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An efficient method for stable transfection of mouse myogenic C2C12 cell line using a nonviral transfection approach
- 1. Hacettepe Univ, Fac Med, Dept Med Biol, TR-06100 Ankara, Turkey
Description
Aim: To describe in detail the use of the T-REx (TM) System (a tetracycline-regulated mammalian expression system; Invitrogen) for rapid, easy, and effective generation of stable C2C12 cell clones. Tetracycline-inducible stable cell lines are useful tools to study the function of various genes in different types of mammalian cells. However, the generation of stable cell lines is a time-consuming, technically difficult, and expensive process.
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