Published January 1, 2011 | Version v1
Journal article Open

Survey and genetic diversity of phytoplasmas from the 16SrV-C and -D subgroups in Hungary

  • 1. FITOLAB Plant Pest Diagnost & Advisory Ltd, Budapest, Hungary
  • 2. Univ Bordeaux, INRA, UMR Fruit Biol & Pathol 1332, F-33883 Villenave Dornon, France

Description

In order to evaluate the risk represented by the wild reservoir as a possible source of 'flavescence doree' outbreaks in Hungary, diverse wild perennial plants growing in vineyard areas were tested for the presence of 16SrV-C and D subgroup phytoplasmas. 16SrV phytoplasmas were detected by nested PCR-RFLP on the 16SrDNA in alders (86% infected) and in clematis (71% infected). Further characterisation by sequencing of the map gene revealed in both plants strains having the same map gene sequence as 'flavescence doree' strains.

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