Yayınlanmış 1 Ocak 2013
| Sürüm v1
Dergi makalesi
Açık
Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris
Oluşturanlar
- 1. Middle E Tech Univ, Dept Biotechnol, Grad Sch Nat & Appl Sci, TR-06800 Ankara, Turkey
- 2. Middle E Tech Univ, Dept Chem Engn, Ind Biotechnol & Metab Engn Lab, TR-06800 Ankara, Turkey
- 3. Ankara Univ, Biochem React Engn Lab, Dept Chem Engn, TR-06100 Ankara, Turkey
Açıklama
BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.
Dosyalar
bib-8892d8e7-0960-434b-b0be-19b6a640e8de.txt
Dosyalar
(309 Bytes)
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