Published January 1, 2013
| Version v1
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Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters
Creators
- 1. Selcuk Univ, Fac Vet, Dept Reprod & Artificial Inseminat, Konya, Turkey
- 2. Pamukkale Univ, Fac Med, Dept Histol & Embriyol, Denizli, Turkey
- 3. Yuzuncu Yil Univ, Fac Med, Dept Med Biol, Van, Turkey
- 4. Selcuk Univ, Fac Vet, Dept Biochem, Konya, Turkey
- 5. Agr Sci & Vet Med Univ, Fac Vet Med, Dept Anim Reprod, Cluj Napoca, Romania
- 6. Ankara Univ, Fac Vet, Dept Pharmacol & Toxicol, TR-06100 Ankara, Turkey
- 7. Selcuk Univ, Fac Sci, Dept Chem, Konya, Turkey
Description
The aim of this study was to determine the effects of raffinose and hypotaurine on sperm parameters after the freeze-thawing of Merino ram sperm. Totally 40 ejaculates of five Merino ram were used in the study. Semen samples, which were diluted with a Tris-based extender containing 10 mM raffinose, 5 mM hypotaurine, 5 mM raffinose +2.5 mM hypotaurine (H + R) and no antioxidant (control), were cooled to 5 degrees C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were then thawed individually at 37 degrees C for 25 s in a water bath for evaluation.
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