Yayınlanmış 1 Ocak 2013 | Sürüm v1
Dergi makalesi Açık

Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant

  • 1. Univ Aberdeen, Rowett Inst Nutr & Hlth, Aberdeen AB21 9SB, Scotland
  • 2. Ondokuz Mayis Univ, Fac Vet Med, Dept Anim Nutr & Nutr Dis, Samsun, Turkey

Açıklama

Bacteria and archaea in frozen (-20 degrees C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected. (C) 2013 Elsevier B.V. All rights reserved.

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