Published January 1, 2013
| Version v1
Journal article
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Comparison of cryoprotective effects of iodixanol, trehalose and cysteamine on ram semen
Creators
- 1. Dicle Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, TR-21280 Diyarbakir, Turkey
- 2. Adiyaman Univ, Fac Med, Dept Med Genet, Adiyaman, Turkey
- 3. Istanbul Univ, Fac Med Vet, Dept Reprod & Artificial Inseminat, TR-34320 Istanbul, Turkey
- 4. Univ Missouri, Coll Vet Med, Dept Vet Pathobiol, Columbia, MO 65211 USA
- 5. Harran Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, Sanliurfa, Turkey
- 6. Lincoln Univ Missouri, Jefferson City, MO 65101 USA
Description
This study was conducted to improve cryosurvival of electroejaculated (EE) ram semen in the presence of iodixanol (OptiPrep (TM)), trehalose or cysteamine. A tris-based extender was used to prepare 12 extenders containing OptiPrep (TM) (Op), trehalose (Tr) or cysteamine (Cy) alone, or different combinations of these compounds. Extenders were designated as follows: Tris (control), Op1.25 (1.25% Op, v/v), Op2.5 (2.5% Op, v/v), Op5 (5% Op, v/v), Tr50 (50 mM Tr), Tr100 (100 mM Tr), Cy (5 mM Cy), OpTr (2.5% Op and 100 mM Tr), OpCy (2.5% Op and 5 mM Cy), TrCy (100 mM Tr and 5 mM Cy), OpTrCy1 (2.5% Op, 100 mM Tr and 5 mM Cy) and OpTrCy2 (1.25% Op, 50 mM Tr and 2.5 mM Cy).
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