Yayınlanmış 1 Ocak 2013
| Sürüm v1
Dergi makalesi
Açık
A novel non-competitive amperometric immunosensor by using thiourea- glutaraldehyde-modified gold electrode for immunoglobulin M detection
Oluşturanlar
- 1. Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey
Açıklama
A novel non-competitive amperometric immunosensor based on a self-assembled monolayer (SAM) of thiourea modified by a polymeric Schiff's base of glutaraldehyde on gold electrode has been developed for determination of IgM. Alkaline phosphatase (ALP)-conjugated monoclonal anti-mouse immunoglobulin M (IgM) antibody was selectively bound to IgM molecules onto the surface of the electrode. Electrochemical response arising from the catalytic reaction of alkaline phosphatase enzyme. Its reaction with various phosphates such as p-aminophenyl phosphate and p-nitrophenyl phosphatase (p-NPP) generates the electrochemically active products p-aminophenol (p-AP) and p-nitrophenol (p-NP), respectively.
Dosyalar
bib-805de3f4-e516-4b3a-b2b8-8881864297d0.txt
Dosyalar
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