Dergi makalesi Açık Erişim

Breeding Doubled Haploid Maize Inbred Lines for Methionine and Lysine Amino Acid Composition

2019    Erdal, Sekip; Cengiz, Rahime; Ozturk, Ahmet

Maize protein quality is deficient due to lower lysine and methionine essential amino acid content. Therefore, developing high methionine and lysine hybrids is of importance in maize. In this study, 64 maize source populations were crossed to RWS x RWK-76 haploid inducer genotype in Sakarya, Turkey to produce haploids in 2016 and 2017. Putative haploids were planted in a greenhouse in 2017-2018 winter season in Antalya, Turkey to produce D-0 lines. 115 D-1 lines were selected and planted in 2018 summer field season in Sakarya for further investigation. A total of 65 D-1 doubled haploid (DH) inbred lines were developed after final evaluations. While the majority of the developed lines gave higher values for methionine and lysine content, Ant-QPMDH-39 and Ant-QPMDH-42 lines were observed to have higher amino acid content when compared to parental lines. Statistics related to haploid induction rate, germination rate, misclassification rate and doubled haploid line rate revealed that effect of source populations cannot be ignored in DH line development. Our results showed that DH technology is efficient in line development in terms of special traits in maize in a short breeding time.