Dergi makalesi Açık Erişim

Comparative Production of Rapid Slide Agglutination Test (RSAT) Antigen Used in Serological Diagnosis of Brucella canis in Different Culture Media in the Fermenter

2014    Erdenlig Gurbilek, Sevil; Baklan, E. Ayhan; Aksoy, H. Yavuz; Stack, Judy

In this study, rapid slide agglutination test (RSAT) antigens were produced by using homologous (Brucella canis RM6/66 and a wild B. canis) and heterologous brucella strains (B. abortus 45/20 and B. melitensis B115) for a quick, practical and economic diagnosis of B. canis infection in dogs and humans. All the test strains were grown in three different culture media, namely, trypton liquid media (TLM), brucella broth media (BBM) and brain heart infusion broth media (BHIBM) to compare their optimal growths. The best growth rate was obtained by B. canis RM6/66 strain. B. melitensis B 115 showed the poorest growth in all test media. The culture media that supported the best growth rate in all tested strains was TLM. RSAT antigens from each test strains were produced without any stringy formation and standardised successfully. It was thought that B. canis RM6/66 and/or wild B. canis might be suitable candidate in commercial RSAT antigen production according to the test results supporting the fact that using homotypic strain increases the diagnostic sensitivity. As a conclusion, it was decided that this antigen will be able to used extensively as a part of routine clinical examination in dogs in Turkey. Furthermore, it was considered that to include RSAT in humans as a part of routine brucellosis diagnosis could help to evaluate the disease more accurately.