Dergi makalesi Açık Erişim

New microsatellite and CAPS-microsatellite markers for clarifying taxonomic and phylogenetic relationships within Origanum L.

2014    Ince, Ayse Gul; Karaca, Mehmet; Elmasulu, Safinaz Y.

Several species in the genus Origanum L. are important among culinary herbs in the world and local markets as raw materials in herb and spices, pharmaceutical and cosmetic industries. Microsatellites also known as simple sequence repeats are routinely being utilized in many plant species. However, the use of microsatellites has still been limited in the genus Origanum due to the scarcity of specific primer pairs. Until recently, there were only 13 microsatellite primer pairs used in two species of Origanum. This study reported 30 primer pairs for development of microsatellite and CAPS-microsatellite markers in 8 different Origanum species. Microsatellite and CAPS-microsatellite markers were utilized to test whether they were useful in species identification and phylogenetic studies in 65 individual samples representing 8 Origanum species. Results indicated that these markers were very useful to clarify taxonomic uncertainties within the genus since some of the markers produced species-specific amplification fashions. Reported DNA markers could be useful in Origanum breeding studies to select desirable chemotypes and tracing adulteration in commercial herbal materials.