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Direct conversion of Cytochrome c spectral shifts to fluorescence using photochromic FRET

   Manioglu, Selen; Atis, Muge; Aas, Mehdi; Kiraz, Alper; Bayraktar, Halil

Photochromic fluorescence resonance energy transfer (pcFRET) was used to monitor the redox activity of non-fluorescent heme protein. Venus fluorescent protein was used as a donor where its emission intensity was reversibly modulated by the absorption change of Cytochrome c.

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