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PRECONDITIONING EFFECT OF BAP ON IN VITRO MULTIPLICATION OF MATURE EMBRYO OF LENTIL (LENS CULINARIS MEDIK.)

2017    Khabbazi, Saber Delpasand; Barpete, Surendra; Sancak, Cengiz; Ozcan, Sebahattin

Lentil (Lens culinaris Medik.) is an important legume with high carbohydrate and protein content that is used as main source of food in many countries. Lentil is recalcitrant and difficult to root under in vitro conditions. Therefore, present study aimed to determine the preconditioning effect of Murashige and Skoog (MS) medium containing 10 and 20 mg L-1 benzylaminopurine (BAP), whereas, postconditioning media were contained of dissimilar levels of BAP combination with indole-3-butyric acid (IBA) for in vitro shoot multiplication using mature embryo as explants. Comparing the three culture conditions, the preconditioned embryo explants had significant difference with unconditioned embryo explants in terms of frequency of shoot regeneration (%) and number of shoot per explant. The presence of IBA in culture medium with each increased concentrations of BAP had positively enhanced the number of shoot per preconditioned explant; whereas, shoots length were inhibited variably. The maximum number (16.33) of shoots was recorded on MS medium containing 0.50 mg L-1 BAP + 0.10 mg L-1 IBA at postconditioning level. Root initiation was observed in all MS media containing IBA after the transfer of shoots. The maximum root induction (75.00%) and mean number of 4.35 roots per shoot was observed on MS medium containing 0.10 mg L-1 IBA. The healthy and rooted plants were transferred to glasshouse for acclimatization and exhibited 85% survival. This regeneration protocol may facilitate genetic transformation studies in lentil.