Published January 1, 2009
| Version v1
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The Comparison of Electrochemical Assay and Agarose Gel Electrophoresis for the Determination of DNA Damage Induced by Kainic Acid
Creators
- 1. Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey
- 2. Ege Univ, Fac Pharm, Dept Biochem, TR-35100 Izmir, Turkey
- 3. Ege Univ, Fac Med, Ctr Brain Res, TR-35100 Izmir, Turkey
Description
A possible DNA damage after interaction of kainic acid (KA) with calf thymus double stranded DNA and genomic DNA was herein determined in in vitro and in vivo conditions using; electrochemical assay and agarose gel electrophoresis. The changes in guanine signal were detected as an indicator of DNA damage in genomic DNA samples isolated from 1 or 10 mg/kg KA-treated animals. The decreased levels of guanine signal were found as 29% and 33% by 1 and 10 mg/kg KA treatment when compared to controls, respectively. The results of gel electrophoresis confirmed DNA damage obtained in identical samples by electrochemical method.
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