Preparation and application of naringenin imprinted cryogel membranes for selectively separation of naringenin from natural environment

Naringenin imprinted cryogel membranes (Nar-ICMs) were constructed for the selective separation of naringenin from the natural media. Acrylamide was used as functional monomer, while 2-hydroxyethyl methacrylate was used as co-monomer. Nar-ICMs were subjected to some characterization analyses, e.g., contact angle measurement, swelling tests, FTIR and SEM. Selectivity studies of Nar-ICMs were carried out both in aqueous media and natural orange juice. In selectivity studies, gallic and caffeic acid molecules were used as competitor agents due to their structural similarity to naringenin. Some results obtained as follows: Contact angle values for Nar-ICMs and non-imprinted cryogel membranes (non-ICMs) were found to be 58.5 degrees and 71.8 degrees, respectively. The maximum adsorption capacities of Nar-ICMs and non-ICMs for naringenin were found to be 66.5 and 14 mg/g, respectively at an initial concentration of 2 mg/mL. The q max and K d values of the high-affinity binding sites of the obtained Scatchard plot were found to be 0.126 mmol/g (34.4 mg/g) and 0.16 mM (1.6.10-4 M), respectively. The selectivity of Nar-ICMs for naringenin was found as 1.99 and 2.26 times high when compared to gallic and caffeic acids, respectively. The adsorption of naringenin from natural orange juice with Nar-ICMs was found to be 843 mu g/g.