Dergi makalesi Açık Erişim
Altinok, Ilhan; Ozturk, Rafet Cagri; Ture, Mustafa
Lactococcus garvieae Lg-per was originally isolated from rainbow trout cultured in cages located on the Turkish coast of the Black Sea in 2011. A whole genome sequence of Lg-per was performed in the present study. The complete genome of Lg-per mapped to the reference genomes of L. garvieae (GCF_000269925.1) and Lactococcus petauri (GCF_014830225.1) had a total of 1,694,407 and 1,945,297 base pairs, respectively. Lg-per had 1955 protein-coding genes and 4 rRNA, 46 tRNA and 1 tmRNA operons. The orthoANI value was 98.30% between Lg-per and L. petauri (GCF_014830225.1) and 93.1% between Lg-per and L. garvieae (GCF_000269925.1). A phylogenetic tree generated from the whole genome sequences (WGS) of several Lactococcus species found that L. petauri (GCA 002154895) was closely related to the Lg-per strain with 98% similarity. Although L. garvieae Lg-per was confirmed as L. garvieae based on phenotypical, biochemical and 16S rRNA sequence, WGS of the Lg-per strain revealed that Lg-per was L. petauri. Using a 16S rRNA-based PCR detection approach, Lg-per was misdiagnosed as L. garvieae since its 16S rRNA gene was 99.9% similar to that of L. garvieae strains. Consequently, the 16S rRNA-based PCR detection approach may not be adequate for the identification of the Lactococcus genus. This is the first study to document the presence of L. petauri in Turkiye. L. garvieae isolates should be analysed using WGS since the same issue might occur in other countries.
Dosya adı | Boyutu | |
---|---|---|
bib-82b9084b-d431-4b1e-a9e0-c451ac6e4de0.txt
md5:d7b13ab887810ff1baf32b57758d9f6e |
178 Bytes | İndir |
Görüntülenme | 5 |
İndirme | 3 |
Veri hacmi | 534 Bytes |
Tekil görüntülenme | 5 |
Tekil indirme | 3 |