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Preparation of biocompatible polymeric bio-scaffold as wound healing activity: Characterization and In vivo analysis

2021    Rethinam, Senthil; Alagumuthu, Tamilselvi; Subaramaniyan, Kumaran; Aruni, A. Wilson; Kavukcu, Serdar Batikan

In this study, a combination of collagen (CL), gelatin (GL) and squid ink (SI) were used to produce a biocompatible polymeric bio-scaffold (CL, CL: GL and CL: GL: SI) was examined for wound healing purposes. Biocompatible polymeric bio-scaffold (BPBS) plays an important role in wound care due to their cost-effective, good mechanical properties, bio-compatible and antimicrobial characteristics. Thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FTIR) of the BPBS has showed the heat stability and nature of samples, respectively, and while scanning electron microscopy (SEM) images revealed the blending of CL fiber, GL, and SI pattern interface. BPBS displayed excellent antimicrobial activity against both E.coli and S.aureus. The inhibition zone of BPBS results obtained in 4.57 +/- 0.30 mm against E.coli and 4.74 +/- 0.15 mm against S.aureus. Mechanical characteristics of BPBS shows tensile strength of 20.88 +/- 0.05 (Mpa), 17.74 +/- 0.05 (%) as Elongation at break, 26.31 +/- 0.21 (%) of flexibility and water absorption of 29.80 +/- 0.05 (%). Invitro analysis of CL: GL: SI, displayed 100% biocompatibility and cell viability was found 3rd days. In vivo study using mice by excisional animal model examined the CL: GL: SI scaffold can significantly accelerate the process of wound healing.