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Characterization of the Interaction between Phthalocyanine and Amyloid Fibrils by Surface-Enhanced Raman Scattering (SERS)

   Losytskyy, Mykhaylo; Akbay, Nuriye; Chernii, Svitlana; Avci, Ertug; Chernii, Viktor; Yarmoluk, Sergiy; Culha, Mustafa; Kovalska, Vladyslava

The applicability of surface-enhanced Raman scattering (SERS) on silver nanoparticles for characterization of interaction between the phthalocyanine molecule and amyloid fibril was studied using the axially coordinated Hf phthalocyanine dichloride and fibrils of amyloidogenic protein insulin. The SERS spectra of the phthalocyanine were obtained; the analytical enhancement factor was estimated to be in the range from 4 to 6 x 10(4). An intensity enhancement in its SERS spectra caused by the presence of fibrillar insulin was observed. Deviation of this enhancement with the change of fibrillar insulin concentration was manifested.

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