Published January 1, 2012
| Version v1
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Observing better transfection efficiency in primary cultured VSMCs: Comparison and development of two different protocols
- 1. Akdeniz Univ, Tip Fak, Tibbi Biyokimya Anabilim Dali, Sch Med, TR-07070 Antalya, Turkey
Description
Aim: In this study, we have evaluated the procedure of transient transfection efficiency of vascular smooth muscle cells (VSMCs) with Lipofectamine (Life Technologies) and FuGENE (ROCHE, FuGENE HD Reagent) transfection reagents using the pCH110 eukaryotic assay vector containing the lacZ reporter gene. We also did try these attainments to transfect VSMCs with Ras(N17) DNA and affirmed our findings.
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