1 Ocak 2009 Dergi makalesi Açık Erişim

Kazan, Dilek; Bal, Hulya; Denizci, Aziz Akin; Ozturk, Nurcin Celik; Ozturk, Hasan Umit; Dilgimen, Aydan Salman; Ozturk, Dilek Coskuner; Erarslan, Altan

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Kazan, Dilek</dc:creator>
  <dc:creator>Bal, Hulya</dc:creator>
  <dc:creator>Denizci, Aziz Akin</dc:creator>
  <dc:creator>Ozturk, Nurcin Celik</dc:creator>
  <dc:creator>Ozturk, Hasan Umit</dc:creator>
  <dc:creator>Dilgimen, Aydan Salman</dc:creator>
  <dc:creator>Ozturk, Dilek Coskuner</dc:creator>
  <dc:creator>Erarslan, Altan</dc:creator>
  <dc:date>2009-01-01</dc:date>
  <dc:description>An alkali tolerant Bacillus strain having extracellular serine alkaline protease activity was newly isolated from compost and identified as Bacillus clausii GMBE 22. An alkaline protease (AP22) was 4.66-fold purified in 51.5% yield from Bacillus clausii GMBE 22 by ethanol precipitation and DEAE-cellulose anion exchange chromatography. The purified enzyme was identified as serine protease by LC-ESI-MS analysis. Its complete inhibition by phenylmethanesulfonylfluoride (PMSF) also justified that it is a serine alkaline protease. The molecular weight of the enzyme is 25.4kDa. Optimal temperature and pH values are 60C and 12.0, respectively. The enzyme showed highest specificity to N-Suc-Ala-Ala-Pro-Phe-pNA. The Km and kcat values for hydrolysis of this substrate are 0.347mM and 1141min-1 respectively. The enzyme was affected by surface active agents to varying extents. The enzyme is stable for 2h at 30C and pH 10.5. AP22 is also stable for 5 days over the pH range 9.0-11.0 at room temperature. AP22 has good pH stability compared with the alkaline proteases belonging to other strains of Bacillus clausii reported in the literature.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/94311</dc:identifier>
  <dc:identifier>oai:zenodo.org:94311</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>PREPARATIVE BIOCHEMISTRY &amp; BIOTECHNOLOGY 39(3) 289-307</dc:source>
  <dc:title>Studies on Alkaline Serine Protease Produced by Bacillus clausii GMBE 22</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
</oai_dc:dc>