1 Ocak 2015 Dergi makalesi Açık Erişim

Qureshi, Anjum; Gurbuz, Yasar; Niazi, Javed H.

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  <dc:creator>Qureshi, Anjum</dc:creator>
  <dc:creator>Gurbuz, Yasar</dc:creator>
  <dc:creator>Niazi, Javed H.</dc:creator>
  <dc:date>2015-01-01</dc:date>
  <dc:description>In this paper, we report on in vitro anti-VEGF aptamer selection and the development of a capacitive aptamer-antibody based sandwich assay for sensitive detection of vascular endothelial growth factor165 (VEGF) in human serum. The assay design involves capturing of VEGF protein through two epitopes, one with anti-VEGF aptamer and the other with the antibody. The capacitive sensor was functionalized with anti-VEGF aptamer which first captures the VEGF protein followed by sandwiching with antibody conjugated magnetic beads (MB-Abs). Changes in capacitance was measured using non-Faradaic electrochemical impedance spectroscopy (nFIS) at different AC electrical frequencies (50-300 MHz). The sandwich assay format exhibited enhanced capacitive signal with respect to concentration with a detection range 5 pg mL(-1) to 1 ngmL(-1) of VEGF protein in human serum. Use of anti-VEGF aptamers enabled chemical stability on sensors during and after the chemical coupling on sensors. This work demonstrated the successful application of on-chip aptamer-antibody based sandwich assays for detection of target proteins in real serum sample for early cancer diagnosis. (C) 2014 Elsevier B.V. All rights reserved.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/82473</dc:identifier>
  <dc:identifier>oai:zenodo.org:82473</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>SENSORS AND ACTUATORS B-CHEMICAL 209 645-651</dc:source>
  <dc:title>Capacitive aptamer-antibody based sandwich assay for the detection of VEGF cancer biomarker in serum</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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