1 Ocak 2015 Dergi makalesi Açık Erişim

Qureshi, Anjum; Gurbuz, Yasar; Niazi, Javed H.

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        "affiliation": "Sabanci Univ Nanotechnol Res & Applicat Ctr, TR-34956 Istanbul, Turkey", 
        "name": "Qureshi, Anjum"
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      {
        "affiliation": "Sabanci Univ, Fac Engn & Nat Sci, TR-34956 Istanbul, Turkey", 
        "name": "Gurbuz, Yasar"
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        "affiliation": "Sabanci Univ Nanotechnol Res & Applicat Ctr, TR-34956 Istanbul, Turkey", 
        "name": "Niazi, Javed H."
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    "description": "In this paper, we report on in vitro anti-VEGF aptamer selection and the development of a capacitive aptamer-antibody based sandwich assay for sensitive detection of vascular endothelial growth factor165 (VEGF) in human serum. The assay design involves capturing of VEGF protein through two epitopes, one with anti-VEGF aptamer and the other with the antibody. The capacitive sensor was functionalized with anti-VEGF aptamer which first captures the VEGF protein followed by sandwiching with antibody conjugated magnetic beads (MB-Abs). Changes in capacitance was measured using non-Faradaic electrochemical impedance spectroscopy (nFIS) at different AC electrical frequencies (50-300 MHz). The sandwich assay format exhibited enhanced capacitive signal with respect to concentration with a detection range 5 pg mL(-1) to 1 ngmL(-1) of VEGF protein in human serum. Use of anti-VEGF aptamers enabled chemical stability on sensors during and after the chemical coupling on sensors. This work demonstrated the successful application of on-chip aptamer-antibody based sandwich assays for detection of target proteins in real serum sample for early cancer diagnosis. (C) 2014 Elsevier B.V. All rights reserved.", 
    "doi": "10.1016/j.snb.2014.12.040", 
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      "pages": "645-651", 
      "title": "SENSORS AND ACTUATORS B-CHEMICAL", 
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    "title": "Capacitive aptamer-antibody based sandwich assay for the detection of VEGF cancer biomarker in serum"
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