1 Ocak 2015 Dergi makalesi Açık Erişim

Qureshi, Anjum; Gurbuz, Yasar; Niazi, Javed H.

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/82473</identifier>
  <creators>
    <creator>
      <creatorName>Qureshi, Anjum</creatorName>
      <givenName>Anjum</givenName>
      <familyName>Qureshi</familyName>
      <affiliation>Sabanci Univ Nanotechnol Res &amp; Applicat Ctr, TR-34956 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Gurbuz, Yasar</creatorName>
      <givenName>Yasar</givenName>
      <familyName>Gurbuz</familyName>
      <affiliation>Sabanci Univ, Fac Engn &amp; Nat Sci, TR-34956 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Niazi, Javed H.</creatorName>
      <givenName>Javed H.</givenName>
      <familyName>Niazi</familyName>
      <affiliation>Sabanci Univ Nanotechnol Res &amp; Applicat Ctr, TR-34956 Istanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Capacitive Aptamer-Antibody Based Sandwich Assay For The Detection Of Vegf Cancer Biomarker In Serum</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2015</publicationYear>
  <dates>
    <date dateType="Issued">2015-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/82473</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.snb.2014.12.040</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">In this paper, we report on in vitro anti-VEGF aptamer selection and the development of a capacitive aptamer-antibody based sandwich assay for sensitive detection of vascular endothelial growth factor165 (VEGF) in human serum. The assay design involves capturing of VEGF protein through two epitopes, one with anti-VEGF aptamer and the other with the antibody. The capacitive sensor was functionalized with anti-VEGF aptamer which first captures the VEGF protein followed by sandwiching with antibody conjugated magnetic beads (MB-Abs). Changes in capacitance was measured using non-Faradaic electrochemical impedance spectroscopy (nFIS) at different AC electrical frequencies (50-300 MHz). The sandwich assay format exhibited enhanced capacitive signal with respect to concentration with a detection range 5 pg mL(-1) to 1 ngmL(-1) of VEGF protein in human serum. Use of anti-VEGF aptamers enabled chemical stability on sensors during and after the chemical coupling on sensors. This work demonstrated the successful application of on-chip aptamer-antibody based sandwich assays for detection of target proteins in real serum sample for early cancer diagnosis. (C) 2014 Elsevier B.V. All rights reserved.</description>
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