1 Ocak 2015 Dergi makalesi Açık Erişim

Qureshi, Anjum; Gurbuz, Yasar; Niazi, Javed H.

Citation Style Language JSON

{
  "DOI": "10.1016/j.snb.2014.12.040", 
  "abstract": "In this paper, we report on in vitro anti-VEGF aptamer selection and the development of a capacitive aptamer-antibody based sandwich assay for sensitive detection of vascular endothelial growth factor165 (VEGF) in human serum. The assay design involves capturing of VEGF protein through two epitopes, one with anti-VEGF aptamer and the other with the antibody. The capacitive sensor was functionalized with anti-VEGF aptamer which first captures the VEGF protein followed by sandwiching with antibody conjugated magnetic beads (MB-Abs). Changes in capacitance was measured using non-Faradaic electrochemical impedance spectroscopy (nFIS) at different AC electrical frequencies (50-300 MHz). The sandwich assay format exhibited enhanced capacitive signal with respect to concentration with a detection range 5 pg mL(-1) to 1 ngmL(-1) of VEGF protein in human serum. Use of anti-VEGF aptamers enabled chemical stability on sensors during and after the chemical coupling on sensors. This work demonstrated the successful application of on-chip aptamer-antibody based sandwich assays for detection of target proteins in real serum sample for early cancer diagnosis. (C) 2014 Elsevier B.V. All rights reserved.", 
  "author": [
    {
      "family": "Qureshi", 
      "given": " Anjum"
    }, 
    {
      "family": "Gurbuz", 
      "given": " Yasar"
    }, 
    {
      "family": "Niazi", 
      "given": " Javed H."
    }
  ], 
  "container_title": "SENSORS AND ACTUATORS B-CHEMICAL", 
  "id": "82473", 
  "issued": {
    "date-parts": [
      [
        2015, 
        1, 
        1
      ]
    ]
  }, 
  "page": "645-651", 
  "title": "Capacitive aptamer-antibody based sandwich assay for the detection of VEGF cancer biomarker in serum", 
  "type": "article-journal", 
  "volume": "209"
}