1 Ocak 2019 Dergi makalesi Açık Erişim

Alttas, Savas; Un, Ismail; Barlas, Ibrahim Omer; Ozturk, Ayla Batu; Karagul, Meryem Ilkay

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    <subfield code="a">Evaluation of the Rho A/Rho-kinase pathway in the uterus of the rat model of polycystic ovary syndrome</subfield>
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    <subfield code="a">The aim of this study was to investigate the expression of RhoA/Rho-kinase in the uterus and the effect of Rho-kinase inhibitors on uterine contractions of dehydroepiandrosterone (DHEA) induced polycystic ovary syndrome (PCOS) rats. Forty-four female Sprague-Dawley (21 days old) rats divided into three groups: The control group (n = 14, any procedure was not performed), vehicle group (n = 14, 0.2 ml of sesame oil, subcutaneous injection, 20 days) and PCOS group (n = 16, DHEA 6 mg/100 g in 0.2 ml of sesame oil, subcutaneous injection, 20 days). The myometrium thickness and uterine wet weight were assessed. The mRNA and protein expressions of Rho A, the effect of Rho-kinase inhibitors (fasudil and Y-27632) on KCI, carbachol, and PGF2 alpha induced contractions were evaluated in the uterus. In the PCOS group, the myometrium thickness and uterine wet weight significantly increased compared to the control group and vehicle group. The mRNA expression level and the immunoreactive score of Rho A, ROCK 1, ROCK 2 were similar in all groups. In the PCOS group, KCI, carbachol, and PGF2 alpha induced uterine contractions significantly increased compared to the control group and vehicle group. Fasudil and Y-27632 significantly inhibited KCI, carbachol, and PGF2 alpha induced uterine contractions in all groups. In conclusion, the expression of Rho A, ROCK 1, ROCK 2 not changed although myometrium thickness, uterine wet weight and the contractile responses of uterus increased in the PCOS group. The results suggest that the Rho-kinase inhibitors effectively suppressed increased contractions in the PCOS group they might be potential therapeutic agents.</subfield>
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    <subfield code="u">Mersin Univ, Fac Med, Dept Med Pharmacol, Mersin, Turkey</subfield>
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    <subfield code="u">Mersin Univ, Fac Med, Dept Med Biol &amp; Genet, Mersin, Turkey</subfield>
    <subfield code="a">Barlas, Ibrahim Omer</subfield>
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    <subfield code="a">Ozturk, Ayla Batu</subfield>
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    <subfield code="u">Mersin Univ, Fac Med, Dept Histol &amp; Embryol, TR-33342 Mersin, Turkey</subfield>
    <subfield code="a">Karagul, Meryem Ilkay</subfield>
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    <subfield code="u">Mersin Univ, Fac Med, Dept Histol &amp; Embryol, TR-33342 Mersin, Turkey</subfield>
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    <subfield code="a">10.1016/j.repbio.2019.01.005</subfield>
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