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Polyglycolide-montmorillonite as a novel nanocomposite platform for biosensing applications

Unal, Betul; Yalcinkaya, Esra Evrim; Gumustas, Sila; Sonmez, Burak; Ozkan, Melek; Balcan, Mehmet; Demirkol, Dilek Odaci; Timur, Suna


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    "creators": [
      {
        "affiliation": "Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey", 
        "name": "Unal, Betul"
      }, 
      {
        "affiliation": "Ege Univ, Fac Sci, Dept Chem, TR-35100 Bornova, Turkey", 
        "name": "Yalcinkaya, Esra Evrim"
      }, 
      {
        "affiliation": "Ege Univ, Fac Sci, Dept Chem, TR-35100 Bornova, Turkey", 
        "name": "Gumustas, Sila"
      }, 
      {
        "affiliation": "Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey", 
        "name": "Sonmez, Burak"
      }, 
      {
        "affiliation": "IZTECH Fac Sci, Dept Chem, TR-35340 Urla Izmir, Turkey", 
        "name": "Ozkan, Melek"
      }, 
      {
        "affiliation": "Ege Univ, Fac Sci, Dept Chem, TR-35100 Bornova, Turkey", 
        "name": "Balcan, Mehmet"
      }, 
      {
        "affiliation": "Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey", 
        "name": "Demirkol, Dilek Odaci"
      }, 
      {
        "affiliation": "Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey", 
        "name": "Timur, Suna"
      }
    ], 
    "description": "In catalytic biosensors, the immobilization of biomolecules in a suitable matrix is one of the vital parameters for obtaining improved systems. Clays, which are intercalated with various organic compounds, have a great tendency to develop biosensors with high stability, sensitivity and reproducibility. Herein, a polymer/clay nanocomposite based on natural silicate montmorilonite (Mt) and a biodegradable polymer polyglycolide (PGA) was prepared and characterized by FT-IR, thermogravimetric analysis, differential thermogravimetric analysis and X-ray diffraction. Then, the resulting matrix was used as a fixation matrix for pyranose oxidase (POx), which was selected as a model enzyme. The bioactive layer was fabricated by immobilization of POx on glassy carbon electrodes by means of PGA-Mt and bovine serum albumin. The POx biosensor revealed a good linear range from 0.01 to 0.5 mM glucose with a LOD of 1.2 mM. After the optimization of the working and preparation conditions, characterization studies were performed for glucose detection. Finally, the PGA-Mt/POx biosensor was confirmed to have detected glucose in beverages without needing any sample pre-treatment.", 
    "doi": "10.1039/c7nj01751k", 
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    "journal": {
      "issue": "17", 
      "pages": "9371-9379", 
      "title": "NEW JOURNAL OF CHEMISTRY", 
      "volume": "41"
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      "type": "publication"
    }, 
    "title": "Polyglycolide-montmorillonite as a novel nanocomposite platform for biosensing applications"
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