1 Ocak 2017 Dergi makalesi Açık Erişim

Unal, Betul; Yalcinkaya, Esra Evrim; Gumustas, Sila; Sonmez, Burak; Ozkan, Melek; Balcan, Mehmet; Demirkol, Dilek Odaci; Timur, Suna

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/111096</identifier>
  <creators>
    <creator>
      <creatorName>Unal, Betul</creatorName>
      <givenName>Betul</givenName>
      <familyName>Unal</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Yalcinkaya, Esra Evrim</creatorName>
      <givenName>Esra Evrim</givenName>
      <familyName>Yalcinkaya</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Chem, TR-35100 Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Gumustas, Sila</creatorName>
      <givenName>Sila</givenName>
      <familyName>Gumustas</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Chem, TR-35100 Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sonmez, Burak</creatorName>
      <givenName>Burak</givenName>
      <familyName>Sonmez</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ozkan, Melek</creatorName>
      <givenName>Melek</givenName>
      <familyName>Ozkan</familyName>
      <affiliation>IZTECH Fac Sci, Dept Chem, TR-35340 Urla Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Balcan, Mehmet</creatorName>
      <givenName>Mehmet</givenName>
      <familyName>Balcan</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Chem, TR-35100 Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Demirkol, Dilek Odaci</creatorName>
      <givenName>Dilek Odaci</givenName>
      <familyName>Demirkol</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Timur, Suna</creatorName>
      <givenName>Suna</givenName>
      <familyName>Timur</familyName>
      <affiliation>Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Polyglycolide-Montmorillonite As A Novel Nanocomposite Platform For Biosensing Applications</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2017</publicationYear>
  <dates>
    <date dateType="Issued">2017-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/111096</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1039/c7nj01751k</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">In catalytic biosensors, the immobilization of biomolecules in a suitable matrix is one of the vital parameters for obtaining improved systems. Clays, which are intercalated with various organic compounds, have a great tendency to develop biosensors with high stability, sensitivity and reproducibility. Herein, a polymer/clay nanocomposite based on natural silicate montmorilonite (Mt) and a biodegradable polymer polyglycolide (PGA) was prepared and characterized by FT-IR, thermogravimetric analysis, differential thermogravimetric analysis and X-ray diffraction. Then, the resulting matrix was used as a fixation matrix for pyranose oxidase (POx), which was selected as a model enzyme. The bioactive layer was fabricated by immobilization of POx on glassy carbon electrodes by means of PGA-Mt and bovine serum albumin. The POx biosensor revealed a good linear range from 0.01 to 0.5 mM glucose with a LOD of 1.2 mM. After the optimization of the working and preparation conditions, characterization studies were performed for glucose detection. Finally, the PGA-Mt/POx biosensor was confirmed to have detected glucose in beverages without needing any sample pre-treatment.</description>
  </descriptions>
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