1 Ocak 2017 Dergi makalesi Açık Erişim

Unal, Betul; Yalcinkaya, Esra Evrim; Gumustas, Sila; Sonmez, Burak; Ozkan, Melek; Balcan, Mehmet; Demirkol, Dilek Odaci; Timur, Suna

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{
  "DOI": "10.1039/c7nj01751k", 
  "abstract": "In catalytic biosensors, the immobilization of biomolecules in a suitable matrix is one of the vital parameters for obtaining improved systems. Clays, which are intercalated with various organic compounds, have a great tendency to develop biosensors with high stability, sensitivity and reproducibility. Herein, a polymer/clay nanocomposite based on natural silicate montmorilonite (Mt) and a biodegradable polymer polyglycolide (PGA) was prepared and characterized by FT-IR, thermogravimetric analysis, differential thermogravimetric analysis and X-ray diffraction. Then, the resulting matrix was used as a fixation matrix for pyranose oxidase (POx), which was selected as a model enzyme. The bioactive layer was fabricated by immobilization of POx on glassy carbon electrodes by means of PGA-Mt and bovine serum albumin. The POx biosensor revealed a good linear range from 0.01 to 0.5 mM glucose with a LOD of 1.2 mM. After the optimization of the working and preparation conditions, characterization studies were performed for glucose detection. Finally, the PGA-Mt/POx biosensor was confirmed to have detected glucose in beverages without needing any sample pre-treatment.", 
  "author": [
    {
      "family": "Unal", 
      "given": " Betul"
    }, 
    {
      "family": "Yalcinkaya", 
      "given": " Esra Evrim"
    }, 
    {
      "family": "Gumustas", 
      "given": " Sila"
    }, 
    {
      "family": "Sonmez", 
      "given": " Burak"
    }, 
    {
      "family": "Ozkan", 
      "given": " Melek"
    }, 
    {
      "family": "Balcan", 
      "given": " Mehmet"
    }, 
    {
      "family": "Demirkol", 
      "given": " Dilek Odaci"
    }, 
    {
      "family": "Timur", 
      "given": " Suna"
    }
  ], 
  "container_title": "NEW JOURNAL OF CHEMISTRY", 
  "id": "111096", 
  "issue": "17", 
  "issued": {
    "date-parts": [
      [
        2017, 
        1, 
        1
      ]
    ]
  }, 
  "page": "9371-9379", 
  "title": "Polyglycolide-montmorillonite as a novel nanocomposite platform for biosensing applications", 
  "type": "article-journal", 
  "volume": "41"
}